ABSTRACT
BACKGROUND: The role of NO in neuropathic pain is controversial. The aim of this study was to compare neuropathic pain behavior after a NOS inhibitor or NO donor treatment and to investigate NOS activity in the dorsal root ganglia (DRG) of young and adult rats after spinal nerve injury. METHODS: The effect of L-nitroarginine methylester (L-NAME) or sodium nitroprusside (SNP) on allodynia was measured in a spinal nerve ligation model of neuropathic pain. A NADPH-diaphorase (NADPH-d) histochemistry was performed on the DRG in a spinal nerve ligation model of neuropathic pain. RESULTS: Mechanical allodynia was increased after spinal nerve injury in both young and adult rats, especially more prominent in young rats. The NOS inhibitor, L-NAME, alleviated allodynia after spinal nerve ligation in young rats but not in adults. The NO donor, SNP, aggravated allodynia in a spinal nerve ligation model of neuropathic pain in young rats but not in adult rats. NOS activity increased prominently in the DRG after spinal nerve ligation in young rats in contrast to adult rats. CONCLUSIONS: Severe neuropathic pain behavior may be a result of an increase of NOS activity in the DRG of young rats after spinal nerve ligation, whereas NO production may not be significantly related to neuropathic pain behaviors in a spinal nerve ligation model of adult rats.
Subject(s)
Adult , Animals , Humans , Rats , Diagnosis-Related Groups , Ganglia, Spinal , Hyperalgesia , Ligation , Neuralgia , NG-Nitroarginine Methyl Ester , Nitric Oxide , Nitroprusside , Spinal Nerves , Tissue DonorsABSTRACT
The Treacher-Collins syndrome is a rare congenital anomaly characterized by mandibular, maxillary, and malar bone hypoplasia, bilateral deformities of auricles, lower lid defects, and antimongoloid slant of the palpebral fissures. The syndrome is associated with considerable difficulty in airway management during anesthesia; difficult, often impossible, endotracheal intubation and face mask ventilation. We report case of Treacher-Collins syndrome in 13-year-old girl who was intubated with fiberoptic bronchoscope and discuss anesthetic consideration.
Subject(s)
Adolescent , Female , Humans , Airway Management , Anesthesia , Bronchoscopes , Congenital Abnormalities , Intubation , Intubation, Intratracheal , Masks , Ventilation , ZygomaABSTRACT
To study the changes in the subsets of peripheral blood T-lymphocytes before and after nephrectomy(Nx) and interferon (IFN) therapy, twenty-four patients with renal cell carcinoma (RCC), who were received radical Nx in all, were divided into three groups; one group consisted of 5 patients with lung metastasis who had been administrated IFN-alpha(M1 group), another group consisted of 10 patients without metastasis who had been administrated IFN-alpha(MO group), and the other group consisted of 9 patients without metastasis who had not been administrated IFN-alpha(IFN(-) group). Immunological parameters such as the percentage of CD3, CD4,CD8 positive cells and the ratio of percentage of CD4/CD8 positive cells were examined by two color flow cytometry. The value of CD4 positive cells showed a significant increase after Nx and the administration of IFN-alpha(P<0.05). The value of CD3 and CD8 positive cells showed no particular changes in all groups. The ratio of percentage of CD4/CD8 positive cells showed a tendency to increased after Nx and administration of IFN-alpha. There was a correlation between the administration of IFN-a and the changes of percentage of CD4 positive cells. We suggest that the administration of IFN-a lead to the increase of helper T cells and immunotherapy such as IFN in RCC patients has some benefits on cellular immunity.